Effects on cell proliferation and EPR spin trapping studies of Abnormal Savda Munziq and its chromatographic subfractions

Methods In addition to the beneficial therapeutical effects of ASMq we also expected possible toxic effects and therefore focussed our in vitro investigations on inhibition of cell proliferation and also investigated its free radical scavenging as well as generating properties using electron spin resonance spectroscopy (ESR). ASMq was tested as aqueous solution as well as different subfractions obtained by sequential extraction with different solvents, i.e. n-hexane, methylene chloride, ethyl acetate, ethanol, methanol, methanol/water and finally water. Furthermore, the ethanolic fraction (containing the highest mass) was subsequently divided into chromatographic subfractions using semi-preparative HPLC. The whole aqueous and organic extracts as well as the ethanolic subfractions were subjected to determination of cell proliferative activity of murine hybridoma cells (YAC-1), which were treated with increasing concentrations of the respective extracts.